Journal: Viruses
Article Title: Recombination and Mutation in a New HP-PRRSV Strain (SD2020) from China
doi: 10.3390/v15010165
Figure Lengend Snippet: Recombination analyses on SD2020 genome. ( A ) Recombination analysis was conducted and a similarity map was generated by Simplot 3.5.1 software with window size of 200 bp and step size of 20 bp. SD2020 as query sequence, was used in similarity plot analysis. ( B ) Schematic diagram of SD2020 PRRSV genomes. ( C ) Recombination regions were shown with segments of different colors and the four recombination breakpoints were detected by RDP v4.1016. Red segments represent the sequence of VR-2332 strain. Blue segment represents the sequence of JXA1-P80 strain. Green segment represents the sequence of JXA1 strain. ( D ) Individual phylogenies reconstructed from non-recombinant fragments identified by RDP v4.1016, with the JXA1-like strain labelled with a blue background and the VR2332 strain labelled with a red background. The phylogenetic tree was constructed by using the neighbor-joining method with 1000 bootstrap replicates in the PHYLIP 3.65 software package.
Article Snippet: The recombination analysis (similarity plot in SIMPLOT v3.5.1) supported that the SD2020 has a recombinant genome ( A).
Techniques: Generated, Software, Sequencing, Recombinant, Construct